Resume
PROFESSIONAL EXPERIENCE
JUN 2019 – Present
RESEARCH ASSOCIATE, STRAIN ENGINEERING – WILLOW BIOSCIENCES
- Metabolic engineering of yeast to produce API chemicals.
- Molecular biology tools development for strain engineering.
- Reviewed and created SOPs to ensure consistency in company’s research and development
- Performed high throughput screening using automated instruments like Bio-Mek, Tecan Fluent, QPix400, Viafill, Agilent Bravo, Echo 550
- Performed a wide range of QA and QC to ensure strains integrity
JAN 2018 – MAY 2019
RESEARCH ASSOCIATE I - ANTHEIA BIO
- Metabolic engineering of yeast to produce alkaloids and other active pharmaceutical ingredients chemicals
- Designed primers, build constructs for donor DNA and use CRISPR engineering to perform yeast genome editing and cloning
- Optimized assay and media conditions for development, bacterial/yeast transformations, performed high-throughput screening with Bio-Mek, and QPix 400
AUG 2016 – JUN 2017
RESEARCH ASSOCIATE INTERN - PROSPECT BIO
- Prepared samples, screened library and performed cloning
- Performed bacterial transformations via electroporation using Bio-Rad Gene Pulser to transform fosmids DNA by applying a high voltage into suspension of competent cells in a cuvette to induce pores expansion.
- Executed experiments to test the specificity of bio-sensors at different chemical concentrations
- Assisted and analyzed experimental data using R programming to determine the fold changes in the responses of bio-sensors
SEP 2012 – MAY 2014
RESEARCH ASSISTANT - SAN JOSE STATE UNIVERSITY
- Analyzed and performed Polymerase Chain Reactions for site-directed mutagenesis for blue/white screening assay of LacZ operons.
- Aided professor in performing DNA purification and precipitation, gel-electrophoresis and analysis of DNA bands.
- Troubleshoot Polymerase Chain Reactions.
EDUCATION
2019-2020
MASTER OF SCIENCE (MS) IN DATA SCIENCE
BELLEVUE UNIVERSITY
2011 - 2016
BACHELOR OF SCIENCE (BS) IN MOLECULAR BIOLOGY, CHEMISTRY MINOR
SAN JOSE STATE UNIVERSITY
Wet lab Skills
- High-throughput screening
- PCR
- Mutagenesis (SDM/SSM)
- Primers design
- DNA/RNA purification
- Gel Electrophoresis and Extraction
- CRISPR
- Cloning
- Bacterial/yeast transformations
- Yeast metabolic engineering
Data Science Skills
- Python
- R
- Machine Learning: KNN, Random Forests, Decision Trees, Logistic Regression, Linear Regression
- Deep Learning: CNN
- SQL
- EDA